Resolution of Two Substrate-Binding Sites in an Engineered Cytochrome P450eryF Bearing a Fluorescent Probe

To elucidate the mechanisms of cooperativity of cytochrome P450eryF an SH-reactive fluorescent probe was introduced close to the substrate-binding site. Cys-154, the only accessible cysteine, was eliminated by site-directed mutagenesis, and a novel cysteine was substituted for Ser-93 in the B′/C loo...

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Bibliographic Details
Main Authors: Davydov, Dmitri R., Botchkareva, Alexandra E., Davydova, Nadezhda E., Halpert, James R.
Format: Artigo
Language:Inglês
Published: Biophysical Society 2005
Subjects:
Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366542/
https://ncbi.nlm.nih.gov/pubmed/15834000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.058479
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