Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome

The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells an...

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Bibliographic Details
Main Authors: Mahony, Timothy J., McCarthy, Fiona M., Gravel, Jennifer L., West, Lani, Young, Peter L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Replication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC136292/
https://ncbi.nlm.nih.gov/pubmed/12050379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.76.13.6660-6668.2002
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