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Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase

In order to find small RNA molecules that are specific and high-affinity ligands of nonstructural 5B (NS5B) polymerase, we screened by SELEX (systematic evolution of ligands by exponential amplification) a structurally constrained RNA library with an NS5BΔC55 enzyme carrying a C-terminal biotinylati...

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Detalhes bibliográficos
Main Authors: Biroccio, Antonino, Hamm, Jörg, Incitti, Ilario, De Francesco, Raffaele, Tomei, Licia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC136087/
https://ncbi.nlm.nih.gov/pubmed/11907208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.76.8.3688-3696.2002
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