Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter for expression...

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Autores principales: Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, Kobayashi, Takatsugu, Kitabatake, Makoto, Takio, Koji, Saito, Kazuki, Shirouzu, Mikako, Hirao, Ichiro, Yokoyama, Shigeyuki
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2002
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Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC135798/
https://ncbi.nlm.nih.gov/pubmed/12409460
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