Real-time monitoring of rolling-circle amplification using a modified molecular beacon design
We describe a method to monitor rolling-circle replication of circular oligonucleotides in dual-color and in real-time using molecular beacons. The method can be used to study the kinetics of the polymerization reaction and to amplify and quantify circularized oligonucleotide probes in a rolling-cir...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC135767/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12136114/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf065 |
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