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Direct observation of base-pair stepping by RNA polymerase

During transcription, RNA polymerase (RNAP) moves processively along a DNA template, creating a complementary RNA. Here we present the development of an ultra-stable optical trapping system with ångström-level resolution, which we used to monitor transcriptional elongation by single molecules of Esc...

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Detalhes bibliográficos
Main Authors: Abbondanzieri, Elio A., Greenleaf, William J., Shaevitz, Joshua W., Landick, Robert, Block, Steven M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1356566/
https://ncbi.nlm.nih.gov/pubmed/16284617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature04268
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