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Signal Sequence Mutations as Tools for the Characterization of LamB Folding Intermediates

lamBA23DA25Y and lamBA23YA25Y tether LamB to the inner membrane by blocking signal sequence processing. We isolated suppressors of lamBA23DA25Y and lamBA23YA25Y, all of which mapped within the LamB signal sequence. Most interesting were mutations that changed an amino acid with a strong positive cha...

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Autori principali: Duguay, Amy Rizzitello, Silhavy, Thomas J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC135451/
https://ncbi.nlm.nih.gov/pubmed/12446642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.24.6918-6928.2002
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