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Mycobacterium smegmatis l-Alanine Dehydrogenase (Ald) Is Required for Proficient Utilization of Alanine as a Sole Nitrogen Source and Sustained Anaerobic Growth

NAD(H)-dependent l-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of l-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression...

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Detalhes bibliográficos
Main Authors: Feng, Zhengyu, Cáceres, Nancy E., Sarath, Gautam, Barletta, Raúl G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC135311/
https://ncbi.nlm.nih.gov/pubmed/12193615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.18.5001-5010.2002
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