Heterologous Production of Clostridium cellulovorans engB, Using Protease-Deficient Bacillus subtilis, and Preparation of Active Recombinant Cellulosomes

In cellulosomes produced by Clostridium spp., the high-affinity interaction between the dockerin domain and the cohesin domain is responsible for the assembly of enzymatic subunits into the complex. Thus, heterologous expression of full-length enzymatic subunits containing the dockerin domains and o...

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Pubblicato in:	J Bacteriol
Autori principali:	Murashima, Koichiro, Chen, Chyi-Liang, Kosugi, Akihiko, Tamaru, Yutaka, Doi, Roy H., Wong, Sui-Lam
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134751/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11741846/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.184.1.76-81.2002
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