Heterologous Production of Clostridium cellulovorans engB, Using Protease-Deficient Bacillus subtilis, and Preparation of Active Recombinant Cellulosomes

In cellulosomes produced by Clostridium spp., the high-affinity interaction between the dockerin domain and the cohesin domain is responsible for the assembly of enzymatic subunits into the complex. Thus, heterologous expression of full-length enzymatic subunits containing the dockerin domains and o...

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Detalhes bibliográficos
Main Authors: Murashima, Koichiro, Chen, Chyi-Liang, Kosugi, Akihiko, Tamaru, Yutaka, Doi, Roy H., Wong, Sui-Lam
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC134751/
https://ncbi.nlm.nih.gov/pubmed/11741846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.1.76-81.2002
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