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Identification of 5′ and 3′ cis-Acting Elements of the Porcine Reproductive and Respiratory Syndrome Virus: Acquisition of Novel 5′ AU-Rich Sequences Restored Replication of a 5′-Proximal 7-Nucleotide Deletion Mutant

We here demonstrate the successful engineering of the RNA genome of porcine reproductive and respiratory syndrome virus (PRRSV) by using an infectious cDNA as a bacterial artificial chromosome. Runoff transcription from this cDNA by SP6 polymerase resulted in capped synthetic RNAs bearing authentic...

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Detalhes bibliográficos
Main Authors:	Choi, Yu-Jeong, Yun, Sang-Im, Kang, Shien-Young, Lee, Young-Min
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology 2006
Assuntos:	Genome Replication and Regulation of Viral Gene Expression
Acesso em linha:	https://ncbi.nlm.nih.gov/pmc/articles/PMC1346850/ https://ncbi.nlm.nih.gov/pubmed/16378975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.80.2.723-736.2006
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Identification of 5′ and 3′ cis-Acting Elements of the Porcine Reproductive and Respiratory Syndrome Virus: Acquisition of Novel 5′ AU-Rich Sequences Restored Replication of a 5′-Proximal 7-Nucleotide Deletion Mutant

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