Sequencing-Independent Method To Generate Oligonucleotide Probes Targeting a Variable Region in Bacterial 16S rRNA by PCR with Detachable Primers

Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bacteria in natural environments. We developed a PCR-based approach that allows synthesis of oligonucleotide probes targeting a variable region in the 16S rRNA without prior knowledge of the target seque...

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Hlavní autoři: Bertilsson, Stefan, Cavanaugh, Colleen M., Polz, Martin F.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2002
Témata:
Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC134391/
https://ncbi.nlm.nih.gov/pubmed/12450831
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.12.6077-6086.2002
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