At Least 1 in 20 16S rRNA Sequence Records Currently Held in Public Repositories Is Estimated To Contain Substantial Anomalies

A new method for detecting chimeras and other anomalies within 16S rRNA sequence records is presented. Using this method, we screened 1,399 sequences from 19 phyla, as defined by the Ribosomal Database Project, release 9, update 22, and found 5.0% to harbor substantial errors. Of these, 64.3% were o...

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Main Authors: Ashelford, Kevin E., Chuzhanova, Nadia A., Fry, John C., Jones, Antonia J., Weightman, Andrew J.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2005
נושאים:
Environmental Microbiology
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317345/
https://ncbi.nlm.nih.gov/pubmed/16332745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.7724-7736.2005
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