At Least 1 in 20 16S rRNA Sequence Records Currently Held in Public Repositories Is Estimated To Contain Substantial Anomalies

A new method for detecting chimeras and other anomalies within 16S rRNA sequence records is presented. Using this method, we screened 1,399 sequences from 19 phyla, as defined by the Ribosomal Database Project, release 9, update 22, and found 5.0% to harbor substantial errors. Of these, 64.3% were o...

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Bibliographische Detailangaben
Hauptverfasser: Ashelford, Kevin E., Chuzhanova, Nadia A., Fry, John C., Jones, Antonia J., Weightman, Andrew J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Environmental Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317345/
https://ncbi.nlm.nih.gov/pubmed/16332745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.7724-7736.2005
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