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Segments missing from the draft human genome sequence can be isolated by transformation-associated recombination cloning in yeast

The reported draft human genome sequence includes many contigs that are separated by gaps of unknown sequence. These gaps may be due to chromosomal regions that are not present in the Escherichia coli libraries used for DNA sequencing because they cannot be cloned efficiently, if at all, in bacteria...

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Detalhes bibliográficos
Main Authors: Kouprina, Natalay, Leem, Sun-Hee, Solomon, Greg, Ly, Albert, Koriabine, Maxim, Otstot, John, Pak, Eugene, Dutra, Amalia, Zhao, Shaying, Barrett, J. Carl, Larionov, Vladimir
Formato: Artigo
Idioma:Inglês
Publicado em: 2003
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1315894/
https://ncbi.nlm.nih.gov/pubmed/12634842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.embor.embor766
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