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SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis

BACKGROUND: Site-directed mutagenesis is a widely-used technique for introducing mutations into a particular DNA sequence, often with the goal of creating a point mutation in the corresponding amino acid sequence but otherwise leaving the overall sequence undisturbed. However, this method provides n...

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Autori principali: Evans, Paul M, Liu, Chunming
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1314889/
https://ncbi.nlm.nih.gov/pubmed/16321147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-6-22
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