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Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

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Bibliografiske detaljer
Main Authors: Dřvínek, Pavel, Hrbáčková, Hana, Cinek, Ondřej, Bartošová, Jana, Nyč, Otakar, Nemec, Alexandr, Pohunek, Petr
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC130775/
https://ncbi.nlm.nih.gov/pubmed/12202599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.9.3485-3488.2002
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