Direct Culture-Independent Strain Typing of Burkholderia cepacia Complex in Sputum Samples from Patients with Cystic Fibrosis

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum...

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Bibliographic Details
Main Authors: Drevinek, Pavel, Vosahlikova, Sarka, Dedeckova, Klara, Cinek, Ondrej, Mahenthiralingam, Eshwar
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2010
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2863923/
https://ncbi.nlm.nih.gov/pubmed/20181898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02359-09
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