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Structural rearrangements in single ion channels detected optically in living cells

Total internal reflection fluorescence microscopy was used to detect single fluorescently labeled voltage-gated Shaker K(+) channels in the plasma membrane of living cells. Tetramethylrhodamine (TMR) attached to specific amino acid positions in the voltage-sensing S4 segment changed fluorescence int...

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Detalhes bibliográficos
Main Authors: Sonnleitner, Alois, Mannuzzu, Lidia M., Terakawa, Susumu, Isacoff, Ehud Y.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC130533/
https://ncbi.nlm.nih.gov/pubmed/12228726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.192261499
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