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The Photophysics of Green Fluorescent Protein: Influence of the Key Amino Acids at Positions 65, 203, and 222

The three amino acids S65, T203, and E222 crucially determine the photophysical behavior of wild-type green fluorescent protein. We investigate the impact of four point mutations at these positions and their respective combinations on green fluorescent protein's photophysics using absorption sp...

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Detalhes bibliográficos
Main Authors: Jung, Gregor, Wiehler, Jens, Zumbusch, Andreas
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1305246/
https://ncbi.nlm.nih.gov/pubmed/15613627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.044412
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