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Direct Observation of the Assembly of RecA/DNA Complexes by Atomic Force Microscopy

The formation of the RecA/DNA nucleofilament on nicked circular double stranded (ds) DNA in the presence of ATPγS was studied using the atomic force microscope (AFM) at nanometer resolution. The AFM allowed simultaneous observation of both dsDNA substrate and RecA protein-coated sections such that t...

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Detalhes bibliográficos
Main Authors: Sattin, Bernie D., Goh, M. Cynthia
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304809/
https://ncbi.nlm.nih.gov/pubmed/15315951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.045567
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