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Detecting Rearrangements of Shaker and NaChBac in Real-Time with Fluorescence Spectroscopy in Patch-Clamped Mammalian Cells

Time-resolved fluorescence detection of site-directed probes is a major tool in the investigation of structure-function relationships of voltage-dependent ion channels. However, the technique has been limited so far to the Xenopus-oocyte system making it difficult to study proteins, like, e.g., the...

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Bibliographic Details
Main Authors: Blunck, Rikard, Starace, Dorine M., Correa, Ana M., Bezanilla, Francisco
Format: Artigo
Language:Inglês
Published: Biophysical Society 2004
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304298/
https://ncbi.nlm.nih.gov/pubmed/15189893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.103.034512
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