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Correcting Confocal Acquisition to Optimize Imaging of Fluorescence Resonance Energy Transfer by Sensitized Emission

Imaging of fluorescence resonance energy transfer (FRET) between suitable fluorophores is increasingly being used to study cellular processes with high spatiotemporal resolution. The genetically encoded Cyan (CFP) and Yellow (YFP) variants of Green Fluorescent Protein have become the most popular do...

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Main Authors: van Rheenen, Jacco, Langeslag, Michiel, Jalink, Kees
Formato: Artigo
Idioma:Inglês
Publicado: Biophysical Society 2004
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304099/
https://ncbi.nlm.nih.gov/pubmed/15041688
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