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Force Spectroscopy of the Double-Tethered Concanavalin-A Mannose Bond

We present the measurement of the force required to rupture a single protein-sugar bond using a methodology that provides selective discrimination between specific and nonspecific binding events and helps verify the presence of a single functional molecule on the atomic force microscopy tip. In part...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ratto, Timothy V., Langry, Kevin C., Rudd, Robert E., Balhorn, Rodney L., Allen, Michael J., McElfresh, Michael W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Biophysical Society 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304091/
https://ncbi.nlm.nih.gov/pubmed/15041680
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