Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method...

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Bibliographic Details
Main Authors: Bloustine, J., Berejnov, V., Fraden, S.
Format: Artigo
Language:Inglês
Published: Biophysical Society 2003
Subjects:
Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303485/
https://ncbi.nlm.nih.gov/pubmed/14507724
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