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Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method...

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Bibliografische gegevens
Hoofdauteurs: Bloustine, J., Berejnov, V., Fraden, S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Biophysical Society 2003
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303485/
https://ncbi.nlm.nih.gov/pubmed/14507724
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