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General Methods for Analysis of Sequential “n-step” Kinetic Mechanisms: Application to Single Turnover Kinetics of Helicase-Catalyzed DNA Unwinding

Helicase-catalyzed DNA unwinding is often studied using “all or none” assays that detect only the final product of fully unwound DNA. Even using these assays, quantitative analysis of DNA unwinding time courses for DNA duplexes of different lengths, L, using “n-step” sequential mechanisms, can revea...

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Bibliografski detalji
Glavni autori: Lucius, Aaron L., Maluf, Nasib K., Fischer, Christopher J., Lohman, Timothy M.
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2003
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303449/
https://ncbi.nlm.nih.gov/pubmed/14507688
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