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The Dead-End Elimination Method, Tryptophan Rotamers, and Fluorescence Lifetimes

The Dead-End Elimination method was used to identify 40 low energy microconformations of 16 tryptophan residues in eight proteins. Single Trp-mutants of these proteins all show a double- or triple-exponential fluorescence decay. For ten of these lifetimes the corresponding rotameric state could be i...

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Main Authors: Hellings, Mario, Maeyer, Marc De, Verheyden, Stefan, Hao, Qiang, Damme, Els J. M. Van, Peumans, Willy J., Engelborghs, Yves
格式: Artigo
語言:Inglês
出版: Biophysical Society 2003
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303361/
https://ncbi.nlm.nih.gov/pubmed/12944302
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