Analysis of the Intrinsic Bend in the M13 Origin of Replication by Atomic Force Microscopy

Atomic force microscopy (AFM) has been used to image a 471-bp bent DNA restriction fragment derived from the M13 origin of replication in plasmid LITMUS 28, and a 476-bp normal, unbent fragment from plasmid pUC19. The most probable angle of curvature of the 471-bp DNA fragment is 40–50°, in reasonab...

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Bibliographic Details
Main Authors: Lu, Yongjun, Weers, Brock D., Stellwagen, Nancy C.
Format: Artigo
Language:Inglês
Published: Biophysical Society 2003
Subjects:
Nucleic Acids
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303096/
https://ncbi.nlm.nih.gov/pubmed/12829495
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