Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.

We find that several key endogenous protein structures give rise to intense second-harmonic generation (SHG)-nonabsorptive frequency doubling of an excitation laser line. Second-harmonic imaging microscopy (SHIM) on a laser-scanning system proves, therefore, to be a powerful and unique tool for high...

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Autors principals: Campagnola, Paul J, Millard, Andrew C, Terasaki, Mark, Hoppe, Pamela E, Malone, Christian J, Mohler, William A
Format: Artigo
Idioma:Inglês
Publicat: 2002
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302489/
https://ncbi.nlm.nih.gov/pubmed/11751336
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