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Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy.

We examine the binding of fluorescent ligands to proteins by analyzing the fluctuation amplitude g(0) of fluorescence fluctuation experiments. The normalized variance g(0) depends on the molecular brightness and the concentration of each species in the sample. Thus a single g(0) measurement is not s...

詳細記述

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書誌詳細
主要な著者: Chen, Y, Müller, J D, Tetin, S Y, Tyner, J D, Gratton, E
フォーマット: Artigo
言語:Inglês
出版事項: 2000
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301003/
https://ncbi.nlm.nih.gov/pubmed/10920037
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