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Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy.

We examine the binding of fluorescent ligands to proteins by analyzing the fluctuation amplitude g(0) of fluorescence fluctuation experiments. The normalized variance g(0) depends on the molecular brightness and the concentration of each species in the sample. Thus a single g(0) measurement is not s...

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Hlavní autoři: Chen, Y, Müller, J D, Tetin, S Y, Tyner, J D, Gratton, E
Médium: Artigo
Jazyk:Inglês
Vydáno: 2000
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301003/
https://ncbi.nlm.nih.gov/pubmed/10920037
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