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Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy.

We examine the binding of fluorescent ligands to proteins by analyzing the fluctuation amplitude g(0) of fluorescence fluctuation experiments. The normalized variance g(0) depends on the molecular brightness and the concentration of each species in the sample. Thus a single g(0) measurement is not s...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Chen, Y, Müller, J D, Tetin, S Y, Tyner, J D, Gratton, E
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2000
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301003/
https://ncbi.nlm.nih.gov/pubmed/10920037
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