A fluorescence energy transfer method for analyzing protein oligomeric structure: application to phospholamban.

We have developed a method using fluorescence energy transfer (FET) to analyze protein oligomeric structure. Two populations of a protein are labeled with fluorescent donor and acceptor, respectively, then mixed at a defined donor/acceptor ratio. A theoretical simulation, assuming random mixing and...

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Hlavní autoři: Li, M, Reddy, L G, Bennett, R, Silva, N D, Jones, L R, Thomas, D D
Médium: Artigo
Jazyk:Inglês
Vydáno: 1999
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1300228/
https://ncbi.nlm.nih.gov/pubmed/10233073
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