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Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells
The ability to manipulate the vaccinia virus (VAC) genome, as a plasmid in bacteria, would greatly facilitate genetic studies and provide a powerful alternative method of making recombinant viruses. VAC, like other poxviruses, has a linear, double-stranded DNA genome with covalently closed hairpin e...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2002
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC129459/ https://ncbi.nlm.nih.gov/pubmed/12196634 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.192420599 |
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