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Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells

The ability to manipulate the vaccinia virus (VAC) genome, as a plasmid in bacteria, would greatly facilitate genetic studies and provide a powerful alternative method of making recombinant viruses. VAC, like other poxviruses, has a linear, double-stranded DNA genome with covalently closed hairpin e...

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Bibliographic Details
Main Authors: Domi, Arban, Moss, Bernard
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2002
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC129459/
https://ncbi.nlm.nih.gov/pubmed/12196634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.192420599
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