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Purification and electron microscopic visualization of functional human spliceosomes
Pre-mRNA splicing takes place in a large and highly dynamic complex known as the spliceosome. Here we report the optimization of a maltose-binding protein (MBP) affinity-purification method to isolate functional spliceosomes for electron microscopic analysis. Visualization of the spliceosome prepara...
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| Hoofdauteurs: | , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
National Academy of Sciences
2002
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC129422/ https://ncbi.nlm.nih.gov/pubmed/12215496 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.182427099 |
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