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Purification and electron microscopic visualization of functional human spliceosomes

Pre-mRNA splicing takes place in a large and highly dynamic complex known as the spliceosome. Here we report the optimization of a maltose-binding protein (MBP) affinity-purification method to isolate functional spliceosomes for electron microscopic analysis. Visualization of the spliceosome prepara...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Zhou, Zhaolan, Sim, Jeonggu, Griffith, Jack, Reed, Robin
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 2002
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC129422/
https://ncbi.nlm.nih.gov/pubmed/12215496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.182427099
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