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Creating New Genes by Plasmid Recombination in Escherichia coli and Bacillus subtilis
Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mu...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1287717/ https://ncbi.nlm.nih.gov/pubmed/16269814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.11.7607-7609.2005 |
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