Preparation, Characterization, and Optimization of an In Vitro C(30) Carotenoid Pathway

The ispA gene encoding farnesyl pyrophosphate (FPP) synthase from Escherichia coli and the crtM gene encoding 4,4′-diapophytoene (DAP) synthase from Staphylococcus aureus were overexpressed and purified for use in vitro. Steady-state kinetics for FPP synthase and DAP synthase, individually and in se...

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Main Authors: Ku, Bosung, Jeong, Jae-Cheol, Mijts, Benjamin N., Schmidt-Dannert, Claudia, Dordick, Jonathan S.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2005
Assuntos:
Physiology and Biotechnology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1287715/
https://ncbi.nlm.nih.gov/pubmed/16269684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.11.6578-6583.2005
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