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Preparation, Characterization, and Optimization of an In Vitro C(30) Carotenoid Pathway
The ispA gene encoding farnesyl pyrophosphate (FPP) synthase from Escherichia coli and the crtM gene encoding 4,4′-diapophytoene (DAP) synthase from Staphylococcus aureus were overexpressed and purified for use in vitro. Steady-state kinetics for FPP synthase and DAP synthase, individually and in se...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2005
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1287715/ https://ncbi.nlm.nih.gov/pubmed/16269684 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.11.6578-6583.2005 |
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