Decreased Electroporation Efficiency in Borrelia burgdorferi Containing Linear Plasmids lp25 and lp56: Impact on Transformation of Infectious B. burgdorferi

The presence of the linear plasmids lp25 and lp56 of Borrelia burgdorferi B31 was found to dramatically decrease the rate of transformation by electroporation with the shuttle vector pBSV2, an autonomously replicating plasmid that confers kanamycin resistance (P. E. Stewart, R. Thalken, J. L. Bono,...

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Veröffentlicht in:	Infect Immun
Hauptverfasser:	Lawrenz, Matthew B., Kawabata, Hiroki, Purser, Joye E., Norris, Steven J.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Bacterial Infections
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC128261/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12183522/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.70.9.4798-4804.2002
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