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Decreased Electroporation Efficiency in Borrelia burgdorferi Containing Linear Plasmids lp25 and lp56: Impact on Transformation of Infectious B. burgdorferi

The presence of the linear plasmids lp25 and lp56 of Borrelia burgdorferi B31 was found to dramatically decrease the rate of transformation by electroporation with the shuttle vector pBSV2, an autonomously replicating plasmid that confers kanamycin resistance (P. E. Stewart, R. Thalken, J. L. Bono,...

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Autori principali: Lawrenz, Matthew B., Kawabata, Hiroki, Purser, Joye E., Norris, Steven J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC128261/
https://ncbi.nlm.nih.gov/pubmed/12183522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.70.9.4798-4804.2002
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