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Resolution of the fluorescence decay of the two tryptophan residues of lac repressor using single tryptophan mutants.

We have studied the time-resolved intrinsic tryptophan fluorescence of the lac repressor (a symmetric tetramer containing two tryptophan residues per monomer) and two single-tryptophan mutant repressors obtained by site-directed mutagenesis, lac W201Y and lac W220Y. These mutant repressor proteins h...

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Hlavní autoři: Royer, C A, Gardner, J A, Beechem, J M, Brochon, J C, Matthews, K S
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1280978/
https://ncbi.nlm.nih.gov/pubmed/2207244
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