Molecular and Biochemical Characterization of the xlnD-Encoded 3-Hydroxybenzoate 6-Hydroxylase Involved in the Degradation of 2,5-Xylenol via the Gentisate Pathway in Pseudomonas alcaligenes NCIMB 9867

The xlnD gene from Pseudomonas alcaligenes NCIMB 9867 (strain P25X) was shown to encode 3-hydroxybenzoate 6-hydroxylase I, the enzyme that catalyzes the NADH-dependent conversion of 3-hydroxybenzoate to gentisate. Active recombinant XlnD was purified as a hexahistidine fusion protein from Escherichi...

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Bibliographische Detailangaben
Hauptverfasser: Gao, Xiaoli, Tan, Chew Ling, Yeo, Chew Chieng, Poh, Chit Laa
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Enzymes and Proteins
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1280293/
https://ncbi.nlm.nih.gov/pubmed/16267294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.22.7696-7702.2005
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