Replacement of Tyrosine 181 by Phenylalanine in Gentisate 1,2-Dioxygenase I from Pseudomonas alcaligenes NCIMB 9867 Enhances Catalytic Activities

xlnE, encoding gentisate 1,2-dioxygenase (EC 1.13.11.4), from Pseudomonas alcaligenes (P25X) was mutagenized by site-directed mutagenesis. The mutant enzyme, Y181F, demonstrated 4-, 3-, 6-, and 16-fold increases in relative activity towards gentisate and 3-fluoro-, 4-methyl-, and 3-methylgentisate,...

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Detalhes bibliográficos
Main Authors: Tan, Chew Ling, Yeo, Chew Chieng, Khoo, Hoon Eng, Poh, Chit Laa
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymes and Proteins
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1272980/
https://ncbi.nlm.nih.gov/pubmed/16237038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.21.7543-7545.2005
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