Purification, Characterization, and Immunogenicity of the Refolded Ectodomain of the Plasmodium falciparum Apical Membrane Antigen 1 Expressed in Escherichia coli

The apical membrane antigen 1 (AMA1) has emerged as a promising vaccine candidate against malaria. Advanced evaluation of its protective efficacy in humans requires the production of highly purified and correctly folded protein. We describe here a process for the expression, fermentation, refolding,...

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Pubblicato in:	Infect Immun
Autori principali:	Dutta, Sheetij, Lalitha, P. V., Ware, Lisa A., Barbosa, Arnoldo, Moch, J. Kathleen, Vassell, Meredith A., Fileta, Bader B., Kitov, Svetlana, Kolodny, Nelly, Heppner, D. Gray, Haynes, J. David, Lanar, David E.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Fungal and Parasitic Infections
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127972/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12011004/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.70.6.3101-3110.2002
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