Purification, Characterization, and Immunogenicity of the Refolded Ectodomain of the Plasmodium falciparum Apical Membrane Antigen 1 Expressed in Escherichia coli

The apical membrane antigen 1 (AMA1) has emerged as a promising vaccine candidate against malaria. Advanced evaluation of its protective efficacy in humans requires the production of highly purified and correctly folded protein. We describe here a process for the expression, fermentation, refolding,...

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Detalhes bibliográficos
Main Authors: Dutta, Sheetij, Lalitha, P. V., Ware, Lisa A., Barbosa, Arnoldo, Moch, J. Kathleen, Vassell, Meredith A., Fileta, Bader B., Kitov, Svetlana, Kolodny, Nelly, Heppner, D. Gray, Haynes, J. David, Lanar, David E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Fungal and Parasitic Infections
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC127972/
https://ncbi.nlm.nih.gov/pubmed/12011004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.70.6.3101-3110.2002
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