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Conformational changes in dynamin on GTP binding and oligomerization reported by intrinsic and extrinsic fluorescence
The effects of guanine nucleotides on the intrinsic and extrinsic fluorescence properties of dynamin were assessed. The intrinsic Trp (tryptophan) fluorescence spectra of purified recombinant dynamin-1 and -2 were very similar, with a maximum at 332 nm. Collisional quenching by KI was weak (∼30%), s...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1276961/ https://ncbi.nlm.nih.gov/pubmed/15954862 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050707 |
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