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Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis

Here we describe the construction and application of six new tagging vectors allowing the fusion of two different types of tagging sequences, epitope and localization tags, to any Bacillus subtilis protein. These vectors are based on the backbone of pMUTIN2 and replace the lacZ gene with tagging seq...

詳細記述

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書誌詳細
主要な著者: Kaltwasser, Marcus, Wiegert, Thomas, Schumann, Wolfgang
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2002
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC127589/
https://ncbi.nlm.nih.gov/pubmed/11976148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.5.2624-2628.2002
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