Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis

Here we describe the construction and application of six new tagging vectors allowing the fusion of two different types of tagging sequences, epitope and localization tags, to any Bacillus subtilis protein. These vectors are based on the backbone of pMUTIN2 and replace the lacZ gene with tagging seq...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Kaltwasser, Marcus, Wiegert, Thomas, Schumann, Wolfgang
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127589/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11976148/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.5.2624-2628.2002
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