Biodegradation of 1,2,3-Trichloropropane through Directed Evolution and Heterologous Expression of a Haloalkane Dehalogenase Gene

Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloropropane (TCP). This highly toxic and recalcitrant compound is a waste product generated f...

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Bibliographic Details
Main Authors: Bosma, Tjibbe, Damborský, Jir̆í, Stucki, Gerhard, Janssen, Dick B.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC126774/
https://ncbi.nlm.nih.gov/pubmed/12089046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.7.3582-3587.2002
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